![]() ![]() Degradation of the JAZ repressors allows the MYC2, MYC3 and MYC4 transcription factors to induce expression of the early genes of JA responses (Lorenzo et al., 2004 Dombrecht et al., 2007 Fernandez-Calvo et al., 2011). In the presence of JA-Ile, JAZ proteins are recruited to the Skip/Cullin/F-box ubiquitin ligase, SCF COI1, and are targeted for degradation in the 26S proteasome (Chini et al., 2007 Thines et al., 2007 Sheard et al., 2010). The COI1 F-box protein acts as a co-receptor with a JAZ protein for the active form of the JA hormone, JA-Ile. JA signaling operates through Jasmonate-ZIM domain proteins (JAZ) that bind to and inhibit the activities of transcription factors, most notably MYC2, MYC3 and MYC4, which are basic helix-loop-helix (bHLH) proteins in the IIIe subfamily that induce the expression of JA-responsive genes (Lorenzo et al., 2004 Dombrecht et al., 2007 Cheng et al., 2011 Fernandez-Calvo et al., 2011 Niu et al., 2011). Jasmonate (JA) is a lipid-derived hormone that induces and controls defense against insects and pathogens, and also has roles in plant growth, development and fertility (Browse, 2009 Kazan and Manners, 2011). Taken together, these results indicate that MYC5, probably together with other, redundant transcription factors, may be activated by JA signaling to induce the expression of MYB21 and components required for male fertility. Importantly, expression of MYB21 and other transcription factors required for stamen and pollen maturation was strongly reduced in stamens of MYC5-SRDX plants relative to the wild type. In particular, MYC5-SRDX plants were male-sterile, with defects in stamen filament elongation, anther dehiscence and pollen viability. Two allelic myc5 mutants exhibited no overt phenotype however, transgenic lines expressing MYC5 fused to an SRDX (SUPERMAN repressive domain X) motif phenocopied mutants defective in JA signaling. A G-box sequence in the JAZ2 promoter was necessary and sufficient for induction by MYC5 (as it is for MYC2, MYC3 and MYC4), and induction of JAZ genes was repressed by co-expression of a stabilized, JAZ1ΔJas repressor. We found that a closely related transcription factor, MYC5 (bHLH28), was able to induce JAZ promoters that control some of the early JA-responsive genes in a Daucus carota (carrot) protoplast expression system. ![]() ![]() MYC2, MYC3 and MYC4 are JAZ-interacting bHLH transcription factors that play a major role in controlling JA responses in vegetative tissue, but are not likely to play a role in reproductive tissue. Arabidopsis thaliana plants deficient in JA-biosynthesis or -signaling are male-sterile, with defects in stamen and pollen development. Question is ,how can i know the source of the problem ? each restart the log empty so i can´t see what cause the problem.Jasmonate hormone (JA) plays critical roles in both plant defense and reproductive development. Question is: i read that many ppl have troubles with the new unraid 6.9.x ,myself included at the beggining. in fact in windows server 2016 everything worked fine ,i just was annoyed by the samba ram cache problem.Īlso when i try to install binhex plex or plex media server oficial ,both show the "action" button ,even if i delete the folders manually. I know the cpu "isnt" supported ,but up to now i didnt have trouble running anything in this machine. The machine is an i7 6700k in a Supermicro X11SSH-F 16GB dd42133 non ECC 2x 9210-8i in IT mode and a lot of HGST drives from 2tb up to 4 tb plus a SSD cache of 500gb and a 120gb ssd drive for the system/appdata/docker/VM on it. This started to happen since i bough the plex Media thing to try the hw encoding. My unraid box hangs completely every time i install/run/try to watch a movie (all of the above). ![]()
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